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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two more splice variants were being documented but thorough nucleotide sequence information is not nevertheless accessible.
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Blocking peptides are peptides that bind exclusively towards the target antibody and block antibody binding. These peptide generally has the epitope acknowledged through the antibody.
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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the best possible final results are...
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This guide outlines even further achievable optimization of mobile and immunoassay parameters to be sure the best possible effects are...
Antibodies bound to the blocking peptide now not bind to the epitope about the concentrate on protein. By comparing the staining from the blocked antibody compared to the antibody on your own, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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